To obtain high-quality, purified nucleic acids, scientists can use several different methods, including phenol-chloroform extraction, cesium chloride/ethidium bromide (CsCl/EtBr) gradient ...
Real-time PCR (qPCR) analysis has become the method of choice for quantitating DNA in a wide variety of applications. The availability of commercial kits has made the technique easy to perform, ...
Morgan Hiebert (left) studies drug-resistant tuberculosis within the National Reference Centre for Mycobacteriology (Winnipeg, Manitoba) led by Hafid Soualhine, situated in the National Microbiology ...
The answer depends on the questions you’re trying to answer, as well as the origin, quantity, and quality of your samples. In this article, we’ll briefly discuss several RNA-seq workflows, how they ...
Starting from whole plant tissues and dissociating cells (protoplasts) or nuclei from the tissue, RNA extraction, cDNA synthesis by reverse transcription, library preparation followed by sequencing, ...
New methods to shape RNA molecules into circles could lead to more effective and long-lasting therapies, shows a study by researchers at the University of California San Diego. The advance holds ...