In typical complementary DNA microarray experiments, two fluorescently labeled RNAs are hybridized to an array of cDNA probes on a glass slide, and their relative gene expression intensities, ratios ...
The main steps involved and limitations of microarray analysis will be encountered. Two stages can be distinguished in microarray experiments: a pre-microarray experiment phase (tissue handling) and ...
Most public-founded large-scale sequencing projects that use cDNAs are primarily using cDNA libraries which are not enriched for full-length cDNAs. Consequently, only a fraction of the resulting ESTs ...
Serial analysis of gene expression (SAGE) uses mRNA from a sample to create complementary DNA (cDNA) fragments for amplification and sequencing using high-throughput sequencing technology. Credit: ...